Florida Museum of Natural History

University of Florida Herbarium (FLAS)

Preparation of Plant Specimens for Deposit as Herbarium Vouchers

A voucher herbarium specimen is a pressed plant sample with collection data deposited for future reference. It supports research work and may be examined to verify the identity of the specific plant used in a study. A voucher specimen must be deposited in a recognized herbarium committed to long-term maintenance. More information on herbaria may be found in our web document "Herbaria and Herbarium Specimens."

Why is voucher material needed? Plant classification is constantly changing. Shifts in species alignments and groupings are made as new evidence comes to light. Identifications are subject to change. Vouchers specimens help cross-reference these changes to previous research.

These steps will help the researcher produce quality specimens acceptable for deposit in a herbarium.

INITIAL PREPARATIONS

Preplanning for the preparation of voucher specimens is crucial.

PRESSING AND DRYING PLANT SPECIMENS

Specimen samples are pressed in a plant press and dried on a plant dryer.

Each specimen sample should consist of a stem with attached leaves and, if at all possible, flowers and/or fruits. The roots of herbaceous plants should also be included. In the case of very large trees, shrubs, or vines, pieces should be selected to illustrate to the greatest extent possible the overall characteristics of the plant and the range of variation in flowers, leaves, and other structures.

Quality specimen data is essential. Do not trust your memory for this information! Assign a unique collection number for each collection, which may be multiple samples of a taxon collected at a single location at the same time. E.g., multiple branches from a tree or shrub or adjacent ones growing together, or multiple individuals of herbaceous plants growing in a colony. This is defined as a Darwin Core Occurrence. Use care to be sure all material collected is actually the same taxonomic entity. Never use the same number twice, even if you go back to the same location to collect the same plant again at a later date or cultivate it in a greenhouse. Data for each collection should be entered in a field notebook (see discussion of label data below) and the number should be written on the folded paper containing the specimen. If ample material is available, a minimum of three specimens should be pressed for each collection, especially if collecting in a region where the flora is poorly known. This will help facilitate the identification of the plants through the distribution of specimens to various herbaria and researchers. An ethical collector will insure that his/her collecting activities do not pose a significant threat to the survival of endangered species or habitats. Ethical herbaria will only accept legally collected specimens. See Florida Plant Collecting : Regulations and Permitting for some guidelines on collecting in Florida.

The plant press consists of a frame (typically wooden, for rigidity), corrugated cardboard ventilators (to allow air to flow through the press), blotter paper (to absorb moisture), and folded paper, typically a newspaper (to contain the plant material). The plant press is tightened using straps with buckles or bolts with wing nuts. The objective of pressing plants is to extract moisture in the shortest period of time, while preserving the morphological integrity of the plant, and to yield material that can be readily mounted on herbarium paper (an acid-free cardstock) for long-term storage.

In order to fit on a standard herbarium sheet, a plant specimen should be pressed flat to no more than 11 X 16 inches. If the specimen will not fit those dimensions, it may be folded or cut into sections. Multiples of smaller plants may be pressed together in order to provide ample material for mounting and study. Small loose pieces, such as seeds, may need to be placed in a small paper packet inside of the newspaper. Large fruits or bulbs are often cut in half lengthwise or in slices prior to pressing. In order to insure rapid and thorough drying, extremely succulent materials such as cactus stems may need to be sliced open and some of the fleshy interior scraped out.

Care should be taken to make good specimens. Pressing material immediately upon collection results in the best specimens. Samples that are allowed to wilt prior to pressing will generally produce inferior specimens. Plants should be carefully arranged as they are placed in the press to maximize preservation of diagnostic features. Leaves, flowers, and fruits should be spread out so that they do not overlap and can be observed from different perspectives. The collection number should be clearly written on the outside of the folded paper containing each plant specimen. The plant press must be kept tight; this prevents shrinkage and wrinkling of the plant material and yields specimens that are easier to mount securely on herbarium paper.

Pressed plants must be thoroughly dried prior to storage and mounting. Best results are obtained with the use of an electric dryer that holds the presses and provides steady bottom heat between 95°F and 113°F (-120°F) (e.g., see Blanco et al., 2006 and the plant dryers and field presses section of the UF Herbarium Plant Specimen Collection and Pressing Bibliography). A low ambient humidity and good airflow around and through the presses also insures rapid and thorough drying of plant material. As the specimens dry, it may be necessary to further tighten the straps on the press to minimize shrinkage and wrinkling. Rapid drying promotes the best retention of plant color, but excessively high temperatures or long drying periods can result in blackened, discolored, and brittle specimens.

Mounting and storage of specimens require a considerable financial commitment in the form of archival materials, labor, and storage cabinets. Herbaria have the prerogative not to accept specimens if the cost of labor/materials for processing is excessive or if the quality of specimens or accompanying data is unsatisfactory. Due to differences in mounting methodologies and materials, most herbaria prefer not to accept already mounted specimens. Because plant classification is generally based on the morphology of flowers and fruits, in most cases sterile (non-flowering or -fruiting) specimens will not be accepted.

IDENTIFICATION OF PLANT SPECIMENS

The identification of plant specimens requires a considerable amount of time and effort. It is important to find out what research is being or has been done on the flora of the region where you are working. A thorough literature review and consultation with herbarium personnel will give you a good basis for starting the identification process.

The identification of unknown plant material is accomplished with the use of dichotomous keys; published plant descriptions, illustrations and photographs; and comparison with properly identified herbarium specimens. A microscope is essential for the observation of many diagnostic features.

Regulations pertaining to collecting plants vary from country to country and state to state, so it is important for you to make official contacts well in advance. It is customary and may be required to deposit one full set of specimens in a herbarium in the host state or country. A local herbarium is the ideal place to begin your quest for identifications, as its collection may be the most comprehensive for the region. It may be possible to arrange to identify your plants and receive assistance from staff members at this institution. But, one must realize that the identification of even relatively common plants may be time-consuming. Most institutions run on tight budgets and do not have staff available to assist or supervise visitors. Even if you are not able to identify your plants to species, you may be able to roughly group them by family or genus. This will allow you to seek experts in specific plant groups who may be willing to look at specimens in their purview. Experts in the flora you are working with may be interested in your collections and willing to give assistance. Your collections may, in fact, be helpful to their projects.

When submitting a plant specimen for identification, it is critical that the sample includes flowers and/or fruits and a portion of the stem with at least several leaves attached. Information of the plant's growth habit, size, and the habitat where it is found (as well as any other features of the plant that may not be apparent from the sample, such as plant color or fragrance) often assist in the identification process. When submitting photos for identification include a general include full-frame close-ups of foliage as well as flowers or fruits. Be sure each photo includes a scale in the form of a ruler or coin. The photos should be accompanied by the same descriptive information provided with a pressed plant sample.

HERBARIUM SPECIMEN LABELS

A plant specimen is incomplete without label data. Label data is a form of observational field data. It must be accurate and descriptive of the single collecting occurrence. The data for the label should meet Darwin Core standards. The following are important elements:

DataDescriptionDarwin Core
Scientific name genus, species, authority, infraspecific information
Determiner the person who identified the plant
Detailed location the location is used by researchers on several levels:
  • for general mapping to region, county or province;
  • for detailed mapping, as in GIS computer applications;
  • to physically locate the plant(s) in order to obtain further research material.
The location should consist of:
country, state or province, county or municipality and a description of the location in reference to roads, road junctions, mile markers and distances from cities and/or towns.
Geographic coordinates are important. Decimal latitude and longitude with the datum used are preferrable. Section, township and range, and/or UTM coordinates may also be given. For more information see: Best Practices for Collecting Geographic Data in the Field
Elevation is also often important.
Origin of material the collection locality for a specimen is always the place where it is growing when your collected it. If you collect material cultivated in a greenhouse or botanical garden, the original source collection data for the material is quite useful and should be included, but clearly distinguished as the origin information. Flowering dates and growth conditions vary considerably when plants are taken into cultivation, especially into a greenhouse.
Habitat the type of plant community where the plant is growing and, if known, a list of associated species.
Frequency is the plant rare, occasional, frequent or common?
Plant description describe characteristics of the plant which are not evident or may be lost upon drying. This includes the plant habit (tree, shrub, vine, herb), height, growth form, flower/fruit color and fragrance, leaf orientation and aroma.
Collector name it is recommended that the collector be consistent and use their full first name, middle initial (or full name) and full last name.
Other collectors other persons present with the collector (*see label examples note below)
Collection number a sequential straightforward numbering system (1,2, 3, ...) is preferable. Complicated schemes cause sorting and searching difficulties in databases.
Date of collection a format with the month spelled out or abbreviated and 4 digit year will prevent confusion. E.g., 3 May 2003, not 3/5/03 or 5/3/03.
Project information the title of the project is important to cross-reference specimens with a project (researcher names, dates and bibliographic data may also be included).
Permit information should be included on the label so there is no doubt the specimen was legally obtained.
Occurrence notes include any information that may be useful to future researchers. E.g., the site was bulldozed or the tree was cut down.

Label Examples

* Please note, label formats vary considerably. We currently recommend that determiner be paired with the identification. There are two standards to denoted multiple collectors and a collection number. E.g.:

Herbarium of the University of Florida, Gainesville, Florida, USA
PLANTS OF FLORIDA

<Scrophulariaceae>
Striga gesnerioides (Willd.) Vatke
   det. D. W. H.

POLK COUNTY: Just S. of Bartow city limits, ± ½ mi. E. of US 17, along S. side of Clear Springs Rd. 2 populations: ± 1800 ft. E. of railroad tracks and ± 45 ft. S. of center line of rd.; ± 400 ft. further E. and 95 ft. S. of center line. Flws. lt. purple; infrequent.

coll. David W. Hall # 1946     17 August 1993
   with Chuck Nance and Allen Ake

Herbarium of the University of Florida, Gainesville, Florida, USA
CULTIVATED PLANTS OF FLORIDA

Nandina domestica Thunb.
   det. K.D.P.

ALACHUA COUNTY: Gainesville, University of Florida campus, cultivated at south end of west side of Rolfs Hall. Cylindrical shrub, ca. 1 m. tall. Fruit bright red.

coll. Kent D. Perkins # 5555     12 Dec 1999

PLANTS OF BORNEO

Artabotrys suaveolens Blume
   Det. J.C. Regalado, 1987

Malaysia. Sabah. Tambunan District: Crocker Range, Km 64.5 on Kota Kinabalu - Tambunan Road. 5°46'N, 116°21'E. Elev. 1220 m. Montane dipterocarp forest. Crocker Formation. Woody climber on roadbank.

John H. Beaman 7178      9 October 1983
With: Reed S. Beaman and Teofile E. Beaman

Herbaria of Michigan State University (MSC) and
Universiti Kebangsaan Malaysia, Sabah Campus (UKMS)
PROJETO FLORA AMAZÔNIA
Instituto Nacional de Pesquisas da Amazônia
The New York Botanical Garden

Rhabdodendron amaconicum (Spr. ex Benth.) Huber

Mun. Óbidos, Pará. 91 km de Oriximiná nos Campos de Ariramba, entre rio Jaramacaru e Igarapé Mutum. Aprox. 01°10'S, 55°35'W. Campina aberta, solo areno pedregoso.
Arbusto de 4 m de altura. Frutos com cálice esverdeados. Fruitos imaturos verdes.

C.A. Cid Ferreira, 9749     04 DEZ 1987


Plantas coletadas com apoio de ENGE-RIO e Mineração Rio Norte, com participação de C.A.C. Ferreira, C. Farney de Sá, G. Martinelli, E. Soares, C.D.A. Mota de E.F. Batista.

Links to More Label Examples

Annotations

Specimens are frequently re-identified once the original label is prepared and/or the specimen has been mounted. These re-identifications are recorded on annotation slips. For more information on annotations, see: Annotation of Herbarium Specimens: Recommentations and Annotation of Type Specimens: Recommentations.

MOUNTING HERBARIUM SPECIMENS

Mounting is the process of affixing a dried pressed plant and its label to a sheet of heavy paper. This provides physical support that allows the specimen to be handled and stored with a minimum of damage. Mounting methods and materials vary widely among herbaria. Most herbaria prefer to have their trained staff prepare specimens with their carefully selected archival materials. Do not mount your specimens before consultation with the depository herbarium.

Because herbarium specimens are intended for long-term study and storage, it is critical that that all supplies used for mounting be both durable and archival. Archival denotes materials that are free of acids and other compounds that may cause them or the specimen to degrade or discolor over time. Consequently, the mounting paper, label paper, packet paper, ink, glue, mounting strips, and storage folders should all be acid free and designed for long-term stability.

Prior to attachment, the specimen and its label are laid out on the paper to allow maximum observation of diagnostic (usually reproductive) features as well as the range of variation in vegetative structures, including both sides of the leaves. Plants are generally positioned in a life-like arrangement (that is, with roots or lower stem toward the bottom of the sheet and flowers toward the top). When laying out the plant, be sure to leave space on the sheet for the specimen label, annotation labels, and institutional accession seal. A paper envelope or packet should also be attached to the sheet to contain any fragments of the specimen that break off over time. Once the optimum arrangement of the specimen has been determined, it is attached to the sheet using a combination of glue and strips of gummed linen cloth tape. Glue is used sparingly to attach the larger portions of the plant, such as stems, large leaves, and fruits. Gummed linen mounting strips are then applied to reinforce portions of the plant that might be torn loose as the specimen is used. Large or bulky items may need to be sewn onto the sheet with a sturdy linen thread. The objective is to secure the specimen firmly to the mounting paper, while leaving some pieces of the plant loose enough to be removed if necessary. Excessive applications of glue that embed flowers and seeds on the sheet may make it impossible to observe diagnostic features or to remove samples, thus rendering the specimen useless for scientific study. The best way to learn proper mounting procedures is through hands-on training and practice with a variety of plant specimens.

The UF Herbarium Specimen Preparation Guide provides an illustrated step-by-step overview of the mounting methods used in the UF Herbarium.

WHERE TO GO FOR MORE INFORMATION

Publications

Web Pages